| Player | # | Pos | Ht | Wt | Age | College |
|---|---|---|---|---|---|---|
| Stanly Arnoux | #53 | LB | 6′ 0″ | 240 | 24 | Wake Forest |
| Adrian Arrington | #87 | WR | 6′ 3″ | 185 | 25 | Michigan |
| K.C. Asiodu | #94 | LB | 6′ 2″ | 225 | 24 | Central Oklahoma |
| Remi Ayodele | #92 | LDT | 6′ 2″ | 318 | 27 | Oklahoma |
| *Joique Bell | #43 | RB | 5′ 11″ | 220 | 24 | Wayne St. |
| Ladell Betts | #46 | RB | 5′ 11″ | 224 | 31 | Iowa |
| Drew Brees+ | #9 | QB | 6′ 0 “ | 209 | 31 | Purdue |
| Alex Brown | #96 | LDE | 6′ 3″ | 260 | 31 | Florida |
| *Charles Brown | #71 | T | 6′ 5″ | 297 | 23 | Southren cal |
| Reggie Bush | #25 | RB | 6′ 0″ | 203 | 25 | USC |
| Jermon Bushrod | #74 | T | 6′ 5″ | 315 | 26 | Towson |
| Jonathan Casillas | #52 | LB | 6′ 1″ | 227 | 22 | Wisconsin |
| Jeff Charleston | #97 | DE | 6′ 4″ | 265 | 27 | Idaho St. |
| Danny Clark | #55 | LLB | 6′ 2″ | 233 | 33 | Illinois |
| Chase Daniel | #24 | QB | 6′ 0″ | 225 | 24 | Missouri |
| Marques Colston | #12 | WR | 6′ 4″ | 225 | 27 | Hofstra |
| *Justin Dresher | #47 | LS | 6′ 1″ | 230 | 23 | Colorado |
| Jo-Lon Dunbar | #56 | LLB | 6′ 0″ | 233 | 25 | Boston College |
| Sedrick Ellis | #98 | RDT | 6′ 1″ | 307 | 25 | USC |
| Heath Evans | #44 | FB | 6′ 0″ | 250 | 32 | Auburn |
| Jahri Evans+★ | #73 | RG | 6′ 4″ | 318 | 27 | Bloomberg |
| *Junior Galette | #93 | DE | 6′ 2″ | 258 | 22 | Stillman |
| Matt Giordano | #37 | S | 5′ 11″ | 207 | 28 | California |
| Randall Gay | #20 | CB | 5′ 11″ | 190 | 28 | LSU |
| Johnathon Goodwin | #76 | C | 6′ 3″ | 318 | 32 | Michigan |
| *Jimmy Graham | #80 | TE | 6′ 6″ | 260 | 24 | Miami(Fla) |
| Jabri Greer | #33 | CB | 5′ 11″ | 180 | 28 | Tennessee |
| Lynell Hamilton | #30 | RB | 6′ 0″ | 235 | 25 | San Diego St. |
| P.J. Hill | #32 | RB | 5′ 10″ | 218 | 24 | Wisconsin |
| Anthoney Hargrove | #69 | DT | 6′ 3″ | 272 | 27 | Georgia Tech |
| Roman Harper+ | #41 | SS | 6′ 1″ | 205 | 28 | Alabama |
| Garrett Hartlely | #5 | K | 5′ 8 “ | 196 | 24 | Oklahoma |
| Devery Henderson | #19 | WR | 5′ 11″ | 200 | 28 | LSU |
| Ramon Humbar | #54 | LB | 5′ 11″ | 232 | 23 | North Dakota St. |
| Tory Humphries | #84 | TE | 6′ 2″ | 255 | 27 | Central Michigan |
| *Chris Ivory | #29 | RB | 6′ 0″ | 222 | 22 | Tiffin |
| Malcolm Jenkins | #27 | FS | 6′ 0″ | 204 | 23 | Ohio St. |
| Jason Kyle | #57 | LS | 6′ 3″ | 242 | 38 | Arizona St. |
| Julius Jones | #21 | RB | 5′ 10″ | 208 | 29 | Notre Dame |
| Marcus Mailei | – | FB | 6′ 0″ | 255 | 24 | Weber St. |
| Robert Meachem | #17 | WR | 6′ 2″ | 210 | 26 | Tennessee |
| Kawika Mitchell | #94 | LB | 6′ 1″ | 253 | 31 | South Florida |
| Marvin Mitchell | #50 | LB | 6′ 3 “ | 249 | 26 | Tennessee |
| Lance Moore | #16 | WR | 5′ 9″ | 190 | 27 | Toledo |
| Thomas Morstead | #6 | P | 6′ 4″ | 225 | 24 | Southern Methodist |
| Carl Nicks+ | #77 | LG | 6′ 5″ | 343 | 25 | Nebraska |
| Tracy Porter | #22 | RCB | 5′ 11″ | 186 | 24 | Indiana |
| Pierson Prioleau | #31 | S | 5′ 11″ | 188 | 33 | Virginia Tech |
| *Patrick Robinson | #34 | CB | 5′ 11″ | 191 | 23 | Florida St. |
| Chris Reis | #39 | FS | 6′ 1″ | 215 | 27 | Georgia Tech |
| Courtney Roby | #15 | WR | 6′ 0″ | 189 | 28 | Indiana |
| Scott Shanle | #58 | RLB | 6′ 2″ | 245 | 31 | Nebraska |
| Darren Sharper | #42 | FS | 6′ 2″ | 210 | 35 | William & Mary |
| Jeremy Shockey | #88 | TE | 6′ 5″ | 251 | 30 | Miami (Fla) |
| Will Smith | #91 | RDE | 6′ 3″ | 282 | 29 | Ohio St. |
| Jon Stinchcomb | #78 | RT | 6′ 5 “ | 315 | 31 | Georgia |
| Zach Strief | #64 | T | 6′ 7″ | 320 | 27 | Northwestern |
| *Matt Tennant | #65 | C | 6′ 4″ | 274 | 23 | Boston College |
| Pierre Thomas | #23 | RB | 5′ 11″ | 215 | 26 | Illinois |
| David Thomas | #85 | TE | 6′ 3″ | 248 | 27 | Texas |
| Leigh Torrence | #24 | CB | 5′ 11″ | 179 | 29 | Stanford |
| Johnathon Vilma+ | #51 | MLB | 6′ 1″ | 230 | 28 | Miami (Fla) |
| Anthony Waters | #59 | LB | 6′ 3″ | 238 | 26 | Clemson |
| Jimmy Wilkerson | #99 | DL | 6′ 2″ | 270 | 30 | Oklahoma |
| DeShawn Wynn | #35 | RB | 5′ 10″ | 232 | 27 | Florida |
| Osama Young | #28 | CB | 6′ 0″ | 186 | 25 | Kent St. |
- * – Rookie
- + – Pro Bowl Selection
- ★- All-Pro Selection